Detection of acute bee paralysis virus by RT-PCR in honey bee and Varroa destructor field samples: rapid screening of representative Hungarian apiaries
نویسندگان
چکیده
منابع مشابه
Molecular detection of Acute Bee Paralysis Virus in Iran
Acute bee paralysis virus (ABPV) is a small single stranded RNA virus recently classified within the family Dicistroviridae, genus Cripavirus. Here, we describe the first study of ABPV in unhealthy bee colonies, which has been an unusual loss in adult bee population and significant honey bee mortality during the year. The aim of this study was evaluation of ABPV infection in honey bee colonies ...
متن کاملFirst molecular detection of Chronic Bee Paralysis Virus (CBPV) in Iran
Among the viruses infecting honey bees, chronic bee paralysis virus (CBPV) is known to induce significant losses in honey bee colonies. CBPV is an unclassified polymorphic single stranded RNA virus. Using RT-PCR, the virus infections in honey bees can be detected in a rapid and accurate manner. Bee samples were collected from 23 provinces of Iran, between July-September 2011 and 2012. A tota...
متن کاملBidirectional Transfer of RNAi between Honey Bee and Varroa destructor: Varroa Gene Silencing Reduces Varroa Population
The mite Varroa destructor is an obligatory ectoparasite of the honey bee (Apis mellifera) and is one of the major threats to apiculture worldwide. We previously reported that honey bees fed on double-stranded RNA (dsRNA) with a sequence homologous to that of the Israeli acute paralysis virus are protected from the viral disease. Here we show that dsRNA ingested by bees is transferred to the Va...
متن کاملmolecular detection of acute bee paralysis virus in iran
acute bee paralysis virus (abpv) is a small single stranded rna virus recently classified within the family dicistroviridae, genus cripavirus. here, we describe the first study of abpv in unhealthy bee colonies, which has been an unusual loss in adult bee population and significant honey bee mortality during the year. the aim of this study was evaluation of abpv infection in honey bee colonies ...
متن کاملGene expression in honey bee (Apis mellifera) larvae exposed to pesticides and Varroa mites (Varroa destructor).
Honey bee (Apis mellifera) larvae reared in vitro were exposed to one of nine pesticides and/or were challenged with the parasitic mite, Varroa destructor. Total RNA was extracted from individual larvae and first strand cDNAs were generated. Gene-expression changes in larvae were measured using quantitative PCR (qPCR) targeting transcripts for pathogens and genes involved in physiological proce...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Apidologie
سال: 2002
ISSN: 0044-8435,1297-9678
DOI: 10.1051/apido:2001004